Reporter Gene Assay for Fish-Killing Activity Produced by Pfiesteria piscicida
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منابع مشابه
Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.
Collaborative studies were performed to develop a functional assay for fish-killing activity produced by Pfiesteria piscicida. Eight cell lines were used to screen organic fractions and residual water fraction by using a 3-[4, 5-dimethylthiazol-(2-4)]-diphenyltetrazolium bromide cytotoxicity assay. Diethyl ether and a residual water fraction were cytotoxic to several cell lines including rat pi...
متن کاملPfiesteria piscicida Bibliography
The presence of P. piscicida in Chesapeake Bay is documented. The appearance and behavior of the algae and symptoms associated with fish mortality were consistent with those previously observed in P. piscicida-associated aquaria fish kills in North Carolina. The discovery of P. piscicida in Chesapeake Bay shows that dinoflagellates have a dramatic and far-reaching impact on fish stocks in shall...
متن کاملPfiesteria piscicida and Dinoflagellates Similar to Pfiesteria
Pfiesteria pisiccida is a microscopic, unicellular organism that is classified as both a mixotrophic and heterotrophic dinoflagellate, which has been associated with both fish deaths and a cause of human illness (Burkholder et al., 1992; Glasgow et al., 1995; Burkholder and Glasgow, 1997). This species possesses a complex life cycle that includes motile forms (e.g. zoospores, gametes, amoebae) ...
متن کاملHormonal activity of polycyclic musks evaluated by reporter gene assay.
Synthetic musk fragrance compounds, such as polycyclic musks (PCMs), are a group of chemicals used extensively as personal care products, and can be found in the environment and the human body. PCMs, such as 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexa-methylcyclopenta-gamma-2-benzopyran (HHCB) and 7-acetyl-1,1,3,4,4,6-hexamethyltetralin (AHTN), are known to have agonistic activities toward human est...
متن کاملDetection and quantification of Pfiesteria piscicida by using the mitochondrial cytochrome b gene.
Mitochondrial cytochrome b was isolated from the dinoflagellate Pfiesteria piscicida, and the utility of the gene for species identification was examined. One of the primer sets designed was shown to be highly specific for P. piscicida. A time step PCR protocol was used to demonstrate the potential of this primer set for quantification of this species.
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ژورنال
عنوان ژورنال: Environmental Health Perspectives
سال: 1999
ISSN: 0091-6765
DOI: 10.2307/3434655